欧罗拉生物科技

离子通道应用报告

离子通道应用报告

使用非放射性分析作为膜蛋白调节剂的筛选工具在科学文献中有很好的记载,并已被广泛用于研究钾通道家族。同样的非放射性Rb+分析原理可以很容易地应用于比目前验证的更多的膜蛋白靶点。以下出版物是ICR应用程序的部分集合,对制药实验室和学术机构都有用。请联系Aurora以获取有关您的特定实验要求的更多信息。 

技术报告

离子通道筛选的非放射性铷流出分析技术

Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels

Georg C. Terstappen

离子通道分析的离子通量和配体结合分析

Ion Flux and Ligand Binding Assays for Analysis of Ion Channels

Georg C. Terstappen

使用ICR8000验证心肌细胞的内源性Na,K,-ATPase的表达

Validation of endogenously expressed Na, K, -ATPase in Cor.At Cardiomyocytes using ICR8000

TRP通道的作用及其作为药物靶点的潜力综述——TRP通道药物发现方法

A Review on the Role of TRP Channelsand Their Potential as Drug Targets_An Insight Into the TRPChannel Drug Discovery Methodologies

应用方向

文献名称

来源

Large Conductance Ca2+– Activated K+ Channels

Functional Analysis of Large Conductance Ca2+ -Activated K + Channels: Ion Flux Studies

Abbott Laboratories

TRP Channels

Rb+ efflux assay for assessment of non-selective cation channel activities

Wyeth Research

Potassium Channels

Potassium Channels Methods and Protocols

SPRINGER

KATP channels

Structure of an open KATP channel reveals tandem PIP2 binding sites mediating the Kir6.2 and SUR1 regulatory interface

Oregon Health & Science University

hERG1 and Kv1.3 Potassium Channels

High throughput clone screening on overexpressed hERG1 and Kv1.3 potassium channels using ion channel reader (ICR) label free technology

University of Florence

NKCC1

Validation of A Robust Label Free Screening Assay for NKCC1 Cotransporter

Aurora 集团

hERG

Design synthesis and biological evaluation of low-toxic lappaconitine derivatives as potential analgesics

西南交通大学

Calcium-Activated Chloride Channel

Development and validation of HTS assay for screening the calcium-activated chloride channel modulators in TMEM16A stably expressed CHO cells

河北医科大学

Cation-Chloride Co-transporter

Development of Rubidium Flux Assay & HTS Campaign for Modulators of a Cation-Chloride Co-transporter

Roche

Na+,K+,-ATPase

Development of an HTS Assay for Na+,K+,-ATPase Using Nonradioactive Rubidium Ion Uptake

河北医科大学

hERG

Evaluation of the Rubidium Efflux Assay for Preclinical Identification of hERG Blockade

Pfizer

hERG

Analogs of MK-499 are differentially affected by a mutation in the S6 domain of the hERG K+ channel

Merck

hERG

Characterization of a hERG Screen Using the IonWorks HT: Comparison to a hERG Rubidium Efflux Screen

Schering-Plough Research Institute

KCNQ2/3

Validation of Rubidium Ion Efflux Assay for KCNQ/M-Channels Using the Ion Channel Reader 8000

Wyeth Research

KCNQ2

A medium-throughput functional assay of KCNQ2 potassium channels using rubidium efflux

AstraZeneca

Nav1.7

Cellular HTS Assays for Pharmacological Characterization of NaV 1.7 Modulators

AstraZeneca

TRPV1

Novel Methodology to Identify TRPV1 Antagonists Independent of Capsaicin Activation

AstraZeneca

Kir6.2

Characterization of polyhormonal insulin-producing cells derived in vitro from human embryonic stem cells

University of British Columbia

Na+-K+-Cl (NKCC1) cotransporter

Blockade of cell volume regulatory protein NKCC1 increases TMZ-induced glioma apoptosis and reduces astrogliosis

University of Pittsburgh Medical Center

Kv10.1 通道

Procyanidin B1, a novel and specific inhibitor of Kv10.1 channel, suppresses the evolution of hepatoma

河北工业大学和河北医科大学

离子通道

A High Throughput Screening Technology-Overcoming Bottlenecks in Ion Channel Drug Targets

Aurora集团

hERG

Rb+ Flux through hERG Channels Affects the Potency of Channel Blocking Drugs: Correlation with Data Obtained Using a High-Throughput Rb+ Efflux Assay

The Society for Biomolecular Screening

离子流与离子通道

Ion Channel Screening

Aurora集团

Rb-ion-efflux-assay

Nonradioactive Rubidium Ion Efflux Assay and Its Applications in Drug Discovery and Development

Discovery Research, Siena, Italy

离子通道

High Throughput Assay Technologies for Ion Channel Drug Discovery

Merck Research Laboratories

离子流

Ion Flux and Ligand Binding Assays for Analysis of Ion Channels

WILEY-VCH Verlag GmbH & Co

KCNQ

Zinc pyrithione-mediated activation of voltage-gated KCNQ potassium channels rescues epileptogenic mutants

Johns Hopkins University

离子通道

Ion Channels: Targets Of High-Tech Screens

Biocompare

Kv1.5

High-throughput analysis of drug binding interactions for the human cardiac channel, Kv1.5

Merck Research Laboratories

Kv1.3

High-Throughput Screening for Kv1.3 Channel Blockers Using an Improved FLIPR-Based Membrane-Potential Assay

Journal of Biomolecular Screening

TRP通道

18 In Vitro and in Vivo Assays for the Discovery of Analgesic Drugs Targeting TRP Channels

Taylor and Francis Group

离子通道

2011 Label Free Tech for DD Chap 8 Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels

University of Siena

钾离子通道

2012 The Antipsychotic Drug Loxapine Is an Opener of the Sodium Activated Potassium Channel Slack

Aurora集团

Slide Helix

Decomposition of Slide Helix Contributions to ATP-dependent

University of British Columbia

p.R1419H-ABCC8

Alternating hypoglycemia and hyperglycemia in a toddler with a homozygous p.R1419H ABCC8

University of British Columbia

NKCC1

A High-Throughput Screening Assay for NKCC1 Cotransporter Using Nonradioactive Rubidium Flux Technology

Aurora集团

KCNQ

Sequence Determinants of subtype-specific actions of KCNQ channel openers

University of Alberta

Kv7

Electrophysiological and pharmacological characterization of a novel and potent neuronal Kv7 channel opener SCR2682 for antiepilepsy

河北医科大学

NKCC1

Role of NKCC1 Activity in Glioma K+ Homeostasis and Cell Growth New Insights With the Bumetanide-Derivative STS66

University of Pittsburgh

Na+,K+,-ATPase

Validation of Rb Uptake Assay

Aurora集团

离子通道

Screening Technologies for ion channel drug discovery

Future Science

新兴应用

导电膜蛋白质在结构和功能上都是多样的。这种异质性是至关重要的服务范围广泛的生理作用。AuroraICR分析满足客户的需求,真诚地努力走在前沿,开拓解决具有挑战性问题的新途径。我们的科学家目前正在与制药和学术合作伙伴合作,开发新的和有效的基于ICR的分析方法,用于转运蛋白、天然化合物和癌症标志物的研究。

转运蛋白Transporters

离子流微弱、电中性限制传统电生理学方法在离子转运体研究中的应用。AuroraICR是一种研究转运体功能和药理的技术。

天然化合物Natural Products

Aurora的离子通道筛选技术能够检测跨膜蛋白质的离子通量。它还能够满足药物开发中动物毒素筛选的高通量需求

癌症标记物Cancer Biomarkers

离子通道参与肿瘤细胞的信号转导。通过使用不产生心律失常的选择性阻滞剂抑制癌细胞中的hERG等通道是抗癌治疗的一种策略。

药物开发Drug Development

离子通道是主要的药物靶点之一,占FDA药物的13%。在新冠肺炎爆发的情况下,有两种针对这些通道的治疗方法。

针对不同类型离子通道的文献

共转运体

钾离子通道

离子通道综述